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Advances in Biochemical Engineering, Volume 010 : by T.K. Ghose ,A. Fiechter, N.Blakebrough

By T.K. Ghose ,A. Fiechter, N.Blakebrough

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Bioteehnol. Bioeng. , Laidler, K. : Biotechnol. Bioeng. 16, 99 (1974) Bunting, P. , Laidler, K. : Biotechnol. Bioeng. 16, 119 (1974) Ngo,T. , Laidler, K. L: Bicchim. Biophys. , Ngo, T. , Laidler, K. : Can. J. Biochem. 53, 1061 (1975) Waterland, L. , Michaels, A. , Robertson, C. : AIChE J. 20, 50 (1974) Marsh,D. , Lee, Y. , Tsao, G. : Biotechnol. Bioeng. : Advan. Catal. 3, 249 (1951) Havewala,N. , Pitcher, W. H. : Biotechnol. Bioeng. 18, 95 (1976) Wilson, E. , Geankoplis, C. : Ind. Eng. Chem. Fundam.

Ii) The whole cell in an encapsulation form that prevents cell reproduction. (iii) Variations on (i) where an additional enzyme, or enzymes, is attached to the surface of the cell which while remaining viable is then incapable of reproduction. (iv) Variations of (i) where due to the manner of flocculation the possibility of reproduction is denied. (v) Cells of the above types where sequences of enzyme reaction are confined by the use of selective inhibitors or inactivated by heat treatment to leave selective enzymic action available.

Thus the extraction process not only would be time consuming but also less efficient. Collagen has been used as a matrix for immobilization of microbial cells by Vieth and his coworkers [54]. For example, heat treated cells ofStreptomyces phaeochromogenes, containing glucose isomerase, have been immobilized on hide collagen in the form of a CH~OH CH~OH S °. C. lunata 0 ~/'''''7"~ ~ ..... OH Biotechnology of Immobilized Multienzyme Systems 47 membrane and demonstrated a high retention of activity at 70 °C.

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