Biotechnology

Bioanalysis and Biosensors for Bioprocess Monitoring by B. Sonnleitner (Editor), T. Scheper (Editor)

By B. Sonnleitner (Editor), T. Scheper (Editor)

The contributions to this certain quantity have been chosen on the way to convey current tendencies within the box of bioprocess tracking and estimation. verified traditional tools are seriously mentioned and non-conventional ones brought. papers concentrate on extra fuzzy techniques corresponding to digital noses or complicated chemometric recommendations. One contribution illustrates the excessive power with the instance of cephalosporin construction. 3 contributions dare to "look" contained in the cells, one through the research of (microscopic) photos, one through attempting to estimate the so-called physiological nation, and the 3rd by means of interpreting the metabolic community. This indicates how refined (bio)chemical in addition to mathematical analytical instruments lead to higher realizing of dwelling platforms and bioprocesses.

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There are no reports currently available of an on-line application of the very powerful MALDI technique (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry). However, this technique has become increasingly important for the analysis of complex molecules and quality control, for example, for glycosylated material [177] or peptides [104] or nucleotides [324]. Of course, mass spectrometry requires expensive equipment. But it should be taken into account that automatic multiplexing of different sample streams is possible and, in addition, a great variety of different substances can be determined simultaneously.

23). Instrumentation of Biotechnological Processes 37 Fig. 23. Schematic design of a biosensor that can be mounted in situ. The biosensor itself sits in a housing and consists of a biocomponent such as one or more immobilized enzymes or cells on top of and in close contact with a suitable type of transducer. A buffer or diluent stream can help to extend the useful dynamic range of the biosensor. The analyte arrives at the biosensor by passing a suitable membrane which enhances selectivity and protects the biosensor.

457] in order to prevent inhibition of methanogenesis: they controlled the addition of the carbon source to a thermophilic anaerobic digestion process. Even linked pO2 , pH and OUR control are reported based on direct mass spectrometric measurements [103, 312]. There are no reports currently available of an on-line application of the very powerful MALDI technique (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry). However, this technique has become increasingly important for the analysis of complex molecules and quality control, for example, for glycosylated material [177] or peptides [104] or nucleotides [324].

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