By Mingyu Wang, Zhonghai Li, Xu Fang (auth.), Feng-Wu Bai, Chen-Guang Liu, He Huang, George T Tsao (eds.)
Cellulolytic Enzyme creation and Enzymatic Hydrolysis for Second-Generation Bioethanol creation, by way of Mingyu Wang, Zhonghai Li, Xu Fang, Lushan Wang und Yinbo Qu Bioethanol from Lignocellulosic Biomass, via Xin-Qing Zhao, Li-Han Zi, Feng-Wu Bai, Hai-Long Lin, Xiao-Ming Hao, Guo-Jun Yue und Nancy W. Y. Ho Biodiesel From traditional Feedstocks, by way of Wei Du und De-Hua Liu setting up Oleaginous Microalgae study types for Consolidated Bioprocessing of solar power, via Dongmei Wang, Yandu Lu, He Huang und Jian Xu Biobutanol, via Hongjun Dong, Wenwen Tao, Zongjie Dai, Liejian Yang, Fuyu Gong, Yanping Zhang und Yin Li Branched-Chain greater Alcohols, through Bao-Wei Wang, Ai-Qin Shi, Ran Tu, Xue-Li Zhang, Qin-Hong Wang und Feng-Wu Bai Advances in Biogas expertise, via Ai-Jie Wang, Wen-Wei Li und Han-Qing Yu Biohydrogen construction from Anaerobic Fermentation, by way of Ai-Jie Wang, Guang-Li Cao und Wen-Zong Liu Microbial gasoline Cells in energy iteration and prolonged purposes, by means of Wen-Wei Li and Guo-Ping Sheng Fuels and chemical compounds from Hemicellulose Sugars, by means of Xiao-Jun Ji, He Huang, Zhi-Kui Nie, Liang Qu, Qing Xu and George T. Tsao
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Extra info for Biotechnology in China III: Biofuels and Bioenergy
1007/s11274-011-0650-5 22 M. Wang et al. 61. Shi Q, Sun J, Yu H et al (2011) Catalytic performance of corn stover hydrolysis by a new isolate Penicillium sp ECU0913 producing both cellulase and xylanase. Appl Biochem Biotechnol 164(6):819–830 62. Montenecourt BS, Eveleigh DE (1977) Semiquantitative plate assay for determination of cellulase production by Trichoderma viride. Appl Environ Microbiol 33(1):178–183 63. Saddler JN (1982) Screening of highly cellulolytic fungi and the action of their cellulase enzyme systems.
However, no natural microorganism is available for commercial production of bioethanol with the CBP strategy. Thus, the development of CBP strains is the core of the CBP process. Currently, both bacterial and yeast species have been explored for this purpose with the following strategies: (1) engineering a cellulase producer to be ethanologenic, and (2) engineering an ethanologen to be cellulolytic . For the first strategy, anaerobic cellulolytic bacteria from the genus Clostridium are good candidates , and the targets for the metabolic engineering of this species include increasing ethanol titer by improving ethanol tolerance through rational designs based on the understanding of the mechanisms underlying its response to ethanol inhibition and random approaches such as the selection of mutants through an evolutionary adaptation procedure, and on the other hand improving ethanol yield by blocking the synthesis of major by-products, as illustrated by the progress with the thermophilic bacterium Thermoanaerobacterium saccharolyticum .
Mobilis to co-ferment pentose and hexose sugars for ethanol production, no commercial applications have been reported to date, due to the incomplete understanding of the species as well as the complexity of industrial substrates, particularly the inhibition of various toxic by-products released during the pretreatment of lignocellulosic biomass and the molecular mechanisms underlying the responses of the species to environmental stresses. With the sequencing of the Z. mobilis genome and elucidation of more functional genes, together with the applications of synthetic and systems biology methodologies [68–71], more efficient strains are expected to be engineered.