Biotechnology in Forage and Turf Grass Improvement by Prof. Dr. Germán Spangenberg, Dr. Zeng-Yu Wang, Prof. Dr.

By Prof. Dr. Germán Spangenberg, Dr. Zeng-Yu Wang, Prof. Dr. Ingo Potrykus (auth.)

This booklet indicates the numerous development made in developing the methodological foundation for the genetic manipulation of forage and turf grasses, with specific emphasis on our most crucial temperate grasses, the fescues and ryegrasses. It presents unique and wonderfully illustrated descriptions of all appropriate methodological points of molecular breeding of forage and turf grasses. the subjects coated variety from the institution of plant regeneration structures from in vitro cultures, the restoration of haploids and somaclonal editions, the combo of complete or partial genomes by means of somatic hybridization, and the construction of transgenic crops, to the advance of molecular markers.

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Extra resources for Biotechnology in Forage and Turf Grass Improvement

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Since a high auxin concentration inhibits embryo germination, levels of auxin are lowered or omitted for plant regeneration. The use of embryos as explants for callus induction, establishment of callus cultures and subsequent plant regeneration is technically preferred. However, due to their zygotic origin, the resulting cultures represent sexual progenies and not clones of performance-tested individuals. The use of explants such as immature inflorescences, leaf bases or roots from mature, outstanding tested plant material is recommended for the establishment of regenerable callus cultures to clonally propagate selected genotypes.

1978, 1982; Dale 1980), root explants (Atkin and Barton 1973; Jackson et al. 1986; Jackson and Dale 1988), internode and peduncle explants (Kasperbauer et al. 1979), immature inflorescences and nodes (Dale et al. 1981; Creemers-Molenaar et al. 1988), meristem tips (Jackson and Dale 1989), and leaf bases and leaf tips (Conger et al. 1982; Joarder et al. 1986; Jackson and Dale 1988, 1989). In most of these studies, modified basal MS media supplemented with 2,4-D were used for callus induction. Plants were regenerated first from callus cultures initiated from cultured immature embryos of L.

Continued Echenique et al. (1996) Park et al. (1990) Park et al. (1990) Park and Walton (1989ab) Rangan (1976) Mohanty et al. (1985) Eapen and George (1989) Eapen and George (1990) Sivadas et al. (1990) Takahashi et al. (1984) Cobb et al. (1985) Sankhla et al. (1992) Talwar and Rashid (1989) Wang and Yan (1984) Cobb et al. (1985) Tyagi et al. (1985) Samantaray et al. (1995) Samantaray et al. (1997) Gonzales and Franks (1987) References ::l 0 ~. ::l. ~ ::l '0""' e:. -< 3~ 0 ::l CL VJ '"~ (D n E..

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